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three months ago i (we) started a project- recombination of the lux operon (which glows in the dark) into E. coli. ergo, making a non glowing bacteria into a glowing bacteria! last week we finished up this project. three months of protocols and gels and DNAses... boiled down to a bunch of bacteria that did not glow for me. :'( even the "sure shot" plasmid to plasmid transformation did not work for me. uhhhhg. maybe sounds like gobbledy gook to you. but to me this is total </3 .
i've been all emo this week. i feel like science hates me.
oh. oh. and also, i've been growing mouse cancer cells (P3 cells) in a flask for tissue culture. it's also been alive for 3 months with no contamination! until, the very last day before i threw it away (and ironically the same day of my lux disappointment). contamination. evil fungus. eeeeeevil. contaminating my kids!
keratin project has not been going well either but that's a whole other story...
and all my professor says to me is "SUCH IS SCIENCE."


sigh. ok.
i'm over it already.
need to stop thinking about science.
ok, how about this-

MY CALENDAAAAARS HAVE ARRIVED!







LISTENING TO: CARPE DIEM by Todor and Emily Haines


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oh who am i kidding.
i am still emo about this.

maybe i didn't use enough ligase? i don't understand. where did the keratin go wrong? and the controls worked so i know the pGEM plasmid was good, the ampicilin was good, the xgal was good, the CIP was good. only the two lux experiments didn't work. whyyyyyyyyyyyy? i really can't get over this. WHYYYYYYY? WEISHEME????? POURQUOI????? AHHHRG!



LISTENING TO: PAPER FLOAT by Cassettes Won't Listen

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